Is d-aspartate produced by glutamic-oxaloacetic transaminase-1 like 1 (Got1l1): a putative aspartate racemase?
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چکیده
منابع مشابه
Aspartate racemase, generating neuronal D-aspartate, regulates adult neurogenesis.
D-aspartic acid is abundant in the developing brain. We have identified and cloned mammalian aspartate racemase (DR), which converts L-aspartate to D-aspartate and colocalizes with D-aspartate in the brain and neuroendocrine tissues. Depletion of DR by retrovirus-mediated expression of short-hairpin RNA in newborn neurons of the adult hippocampus elicits profound defects in the dendritic develo...
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Pig heart “soluble” glutamic-aspartic transaminase (L-aspartate : 2-oxoglutarate aminotransferase, EC 2.6.1.1) will catalyze the exchange of an amino group between glutamate and ketoglutarate without the participation of any other amino acid or keto acid (1). This “exchange transamination” also occurs between aspartate and oxaloacetate. The rates of these reactions are comparable to the physiol...
متن کاملAutomated determination of serum glutamic oxaloacetic transaminase.
A simplified and precise automated method is described for the colorimetric determination of serum glutamic oxaloacetic transaminase. The enzymically generated oxaloacetic acid is dialyzed, coupled with Fast Ponceau L, a diazonium salt, to form a colored solution whose absorbance is measured at 455 m . Dialysis eliminates the need for a serum control determination, thereby halving the analysis ...
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Eighteen patients developed abnormally low serum glutamic oxaloacetic transaminase values during metronidazole therapy. Metronidazole absorbs at 340 nm, simulating reduced nicotinamide adenine dinucleotide, which is the final colorimetric product of the serum glutamic oxaloacetic transaminase assay.
متن کاملDffluoro-oxaloacetate with Aspartate Transaminase
Difluoro-oxaloacetate interacts with the aldimine form of aspartate transaminase to give a complex, the dissociation constant ofwhich has been determined spectrophotometrically and by 19F n.m.r. (nuclear magnetic resonance). The 19F n.m.r. line-width-pH and chemical-shift-pH profiles of difluoro-oxaloacetate in the presence of the aldimine form of the enzyme both show inflexion points in the pH...
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ژورنال
عنوان ژورنال: Amino Acids
سال: 2014
ISSN: 0939-4451,1438-2199
DOI: 10.1007/s00726-014-1847-3